Part:BBa_K3790231:Design

T7 gene product 2 controlled by pBAD/araC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1335
  Illegal SapI site found at 961
  

Design Notes

The following primers were used for constructing this part.

doubleter_f	5-CGATATAAGTTGTAATTCTCaaataataaaaaagccggattaataatctggc
doubleter_r	5-GAAGGCTCTCAAGGGCATtcacactggctcaccttcg
gp2_f		5-GAATGCCCTTGAGAGCCTTCTTACTTCGGTGCTACACAAGGAC
gp2_r		5-gagttaaggaggtaaaaaaaATGTCAAACGTAAATACAGGTTCACTTAG
pBAD_f		5-tttttttacctccttaactctagtagctagcc
pBAD_r		5-TGGTGCTACGCCTGAATAAGttatgacaacttgacggctacatcattc
pLysS_f		5-tagccgtcaagttgtcataaCTTATTCAGGCGTAGCACCAG
pLysS_r		5-GAGAATTACAACTTATATCGTATGGGGC
gp2_coding1 	5-GGGCTACCGTAGAGTCCTCGGAGCATTCCTTCGAGGTTCCAATCTACGCTGAaACCC
gp2_coding2	5-CAGGTTCACTTAGTGTGGACAATAAGAAGTTTTGGGCTACCGTAGAGTCCTC
gp2_coding3	5-ATGTCAAACGTAAATACAGGTTCACTTAGTGTGGACAATAAG
gp2_template1	5-GGAACGTATTGCCATTCGGCTAACTCCAGAGCTTCGTCTAGGGTtTCAGCGTAGATTGG	
gp2_template2	5-CAAGGACGCACACGAGTAACCTCAAAGCCAGCCGGAACGTATTGCCATTCGGC      	
gp2_template3	5-TTACTTCGGTGCTACACAAGGACGCACACGAGTAAC                    
pbad_gp5.7_overlappcr	5-GGTAGTCAGACATtttttttacctccttaactctagtagctagcc	
pbad_gp2_overlappcr	5-TTACGTTTGACATtttttttacctccttaactctagtagctagcc	
f_gp2repair_ins	5-CGTATTGCCATTCGGCTAACTC 	
f_gp2repair_tem	5-CGTTTAAGGGCACCAATAACTGC	
r_gp2repair_ins	5-GTTATTGGTGCCCTTAAACGCC 	
r_gp2repair_tem	5-GTTAGCCGAATGGCAATACGTTC